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All Journal Journal of Tropical Life Science : International Journal of Theoretical, Experimental, and Applied Life Sciences METAMORFOSA Journal of Biological Sciences Bioscience Jurnal Biologi Universitas Andalas Jurnal Riset Kimia Jurnal Penelitian Pendidikan IPA (JPPIPA) Jurnal Hilirisasi IPTEKS (JHI) Eksakta : Berkala Ilmiah Bidang MIPA Majalah Kedokteran Andalas Bioscientist : Jurnal Ilmiah Biologi
Anthoni Agustien
Jurusan Biologi, FMIPA Universitas Andalas Padang
Agriculture, Biological Sciences & Forestry Astronomy Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Chemistry Civil Engineering, Building, Construction & Architecture Computer Science & IT Dentistry Education Energy Engineering Environmental Science Health Professions Immunology & microbiology Materials Science & Nanotechnology Mathematics Mechanical Engineering Medicine & Pharmacology Physics Public Health Social Sciences Veterinary Other

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Journal : Jurnal Penelitian Pendidikan IPA (JPPIPA)

 1 
SKRINING BAKTERI PENGHASIL POLI(3-HIDROKSIBUTIRAT) P(3HB) DARI SAMPEL IKAN KEMBUNG (Rastrelliger sp.) Inayati Rahmi; Anthoni Agustien; Akmal Djamaan
Jurnal Penelitian Pendidikan IPA Vol. 9 No. 10 (2023): October
Publisher : Postgraduate, University of Mataram

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29303/jppipa.v9i10.5262

Abstract

Bioplastic P(3HB) is a plastic that can be decomposed by decomposing microorganisms. Bacteria can produce P(3HB) in conditions of low nutrition and high carbon sources accumulated in cells as energy reserves. Isolation of bacteria-producing bioplastic P(3HB) was carried out in mackerel fish's gills and intestines, allowing direct contact with polluted waters. This research aimed to determine the presence of P(3HB) bioplastic-producing bacteria in mackerel fish samples and to screen for P(3HB) bioplastic-producing bacteria. The stages of the research were the isolation of P(3HB) producing bacteria from the gills and intestines of mackerel fish, screening of P(3HB) bioplastic-producing bacteria by using Nile Blue A staining, and molecular identification of bioplastic-producing bacteria using 16S rRNA gene. The results of bacterial isolation in the gills and intestines of mackerel obtained 10 bacterial isolates. P(3HB) screening found 1 bacterium showing orange fluorescence, namely IKE-1 isolate which was isolated from the gills of mackerel fish and included in the Gram-negative group with a monobacilli cell shape. Molecular identification of bacteria by using the 16S rRNA gene that isolate IKE-1 is Enterobacter roggenkampii. These results indicate that Enterobacter roggenkampii can produce P(3HB) which was confirmed by the screening results
Co-Authors - Nurmiati - Nurmiati - Nurmiati Aghni Aznia Akmal Djamaan Armaleni Armaleni Ayu Putri Ningsih Chairul Chairul Cici Wulandari Desma Yuni Deswati Deswati Devi Syafriyani Dianty Putri Dina Wahyuna Djong Hon Tjong Efrizal Efrizal Efrizal Efrizal Fadhila Cantika Ferry Lismanto Syaiful Feskaharny Alamsjah Fuji Astuti Febria Ikel Fitri Yanis Ilsa Septia Putri Inayati Rahmi Irdawati Irdawati Irfan Mustafa irfan mustafa Jetty Nurhajati Kustiasih Lestari Linar Z. Udin Lola Alviche Melisa Ainil Fajri Mira Rosnawita Nasril Nasir Nasril Nasir Nasril Nasir Nasril Nasir Nasril Nasir Nurhajati, Jetty Nurmiati Nurmiati Nurmiati Nurmiati Periadnadi Periadnadi PINGKAN ADITIAWATI Pingkan Aditiawati Rahmadani Eka Pitri Rahmi Adila Riski Budi Yani Rusnam Rusnam Suwirmen, Suwirmen Syamsuardi Syamsuardi Syukria Ikhsan Zam Syukria Ikhsan Zam Tesri Maideliza Tri Widia Ningsih Alam Uci Mela Sari Udin, Linar Z. Widia Firliani Wira Hastuti Yetria Rilda Yurike Nova Edlin Zakaria, Indra Junadi Zuhri Syam Zuhri Syam